milk santa cruz biotechnology sc 8008 mouse α traf2  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Human colon stem cells are the principal epithelial responders to bacterial antigens

doi: 10.1101/2025.02.07.637053

Figure Lengend Snippet: A. Bulk RNAseq gene set enrichment analysis of upregulated genes of stem cell-enriched organoids. Response genes are enriched for inflammatory pathways. n=2. B. qPCR of CXCL8 of duodenal-derived stem cell-enriched organoid CXCL8 upon microbial exposure can be inhibited with a specific NFκB blocker. Error bars indicate SEM. (unpaired t-test) n=4.

Article Snippet: The slides were blocked for 30 min in 5% BSA at RT and incubated ON at 4°C with primary antibodies (mouse α-NFκB p65 L8F6 1:50 (CST 6956S, Cell signaling Technology) in 5% BSA-PBS).

Techniques: Derivative Assay

Journal: bioRxiv

Article Title: Human colon stem cells are the principal epithelial responders to bacterial antigens

doi: 10.1101/2025.02.07.637053

Figure Lengend Snippet: A . Bulk RNAseq expression levels of genes involved in Toll-Like receptor signaling pathway in five days differentiated enterocytes-enriched organoids versus stem cell-enriched organoids. n=2 B. Immunohistochemistry of NFκB (p65) in stem cell-enriched organoids compared to five days differentiated enterocyte-enriched organoids, shows nuclear translocation of NFκB upon exposure (2h). n=3 C. Quantification of nuclear translocation of NFκB. The number of cells with nuclear NFκB is higher in stem cell-enriched organoids compared to enterocyte-enriched organoids (Mann-Whitney). Error bars indicate SEM. n=3 qPCR: quantitative polymerase chain reaction.

Article Snippet: The slides were blocked for 30 min in 5% BSA at RT and incubated ON at 4°C with primary antibodies (mouse α-NFκB p65 L8F6 1:50 (CST 6956S, Cell signaling Technology) in 5% BSA-PBS).

Techniques: Expressing, Immunohistochemistry, Translocation Assay, MANN-WHITNEY, Real-time Polymerase Chain Reaction